Huck Finn - Jim :: essays research papers

Throughout all of his adventures Jim shows compassion as his most prominent trait. He makes the reader aware of his many superstitions and Jim exhibits gullibility in the sense that he Jim always assumes the other characters in the book will not take advantage of him. One incident proving that Jim acts naive occurs halfway through the novel, when the Duke first comes into the scene "By right I am a duke! Jim’s eyes bugged out when he heard that..." In the novel, Huck Finn, one can legitimately prove that compassion, superstitious and gullibility illustrate Jim’s character perfectly. To begin with, among the many characteristics of Jim, his compassionate nature shows throughout the book. When Huck and Jim come across the floating boathouse, Jim finds a dead man inside. He advises Huck not to look as he says, "It’s a dead man... dead two er three days... come in Huck, but doan’ look at his face." At the end of the book the reader finds out that the dead man turns out as Huck’s father. Further on down the river, Huck and Jim engage in a deep conversation. Jim speaks of the family he feels he has left behind. Jim tries hard to save up all his money in hopes of buying back his wife and children when he becomes a free man. He expresses that he feels terrible for leaving behind his family and misses them very much. As a result, Huck feels responsible and guilty for ruining Jim’s freedom. Huck decides that he wants to reveal the truth, that Jim really isn’t a free man. His conscience tells him not to and instead he fi nds himself helping Jim rather than giving him up. Jim feels so thankful to Huck when he says ". . .it’s all on account of Huck, I’s a free man, ... you’s the best friend Jim’s ever had..." Even further along, Huck becomes separated from Jim and living at the Grangerford’s. Huck doesn’t know if he’ll ever see Jim again. He also doesn’t realize Jim has found a hiding spot not very far away. He asks one of the Grangferford’s slaves about Huck’s condition and how well the lifestyle of the Grangerfords suites him. A slave reunites Jim and Huck and Huck proceeds to ask, "Why didn’t you tell my Jack to fetch me here sooner, Jim?" Jim replies, "Well, ‘twarn’t no use to ‘sturb you, Huck.

Nuclear Waste Management position at Ontario

As a second year student at the University of Toronto, majoring in Environmental Science, I am excited to be applying to the Ontario Power Generation. This is an excellent opportunity to apply my knowledge and skills while gaining practical work experience and further understanding the science behind it. I believe that I would be a great fit given my background, thirst for experience, and never-ending enthusiasm, and I would appreciate the opportunity to contribute this company. Throughout my schooling, as a science student, I have had a great deal of practice in the raw technical skills needed in data management and handing dangerous materials. Laboratories hold a strict code of conduct for safety procedures, and expect quick and efficient work with little error. This has given me acute attention to detail, even with a time limit. My time spent in laboratories has also forced me to gain good understanding of Microsoft office suite and statistics, as many of the reports require manipulation and interpretation of mass amounts of data. Cooperation is also a necessary skill I have perfected in laboratories, as many are performed in pairs; however I also retain the ability to perform alone effectively, and complete my work independently. Also, as a science student, I have a good background understanding of the concepts behind nuclear waste management. In addition to a chemistry, physics, and the three main principles of concentrate and contain, dilute and disperse, and delay and decay, I also understand cultural perspectives and challenges of this field. Throughout my career I have been heavily involved in the community, which has given me a strong work ethic, and excellent interpersonal skills I own today . In High School, I was a part-time cashier, in a fast-paced environment , and in direct interaction with customers . Through this I developed the professionalism and customer service initiative needed to excel in a service job, as well as acute problem solving skills for everything from computer issues to customer complaints, and an efficient work ethic. Perhaps my favourite, and most rewarding experience, however has been my volunteer time at Habitat for Humanity. It was an opportunity to step outside of my comfort zone , and though the work was difficult at first, I kept with it, and was greatly rewarded . I have since maintained a proactive, hands-on, and enthusiastic attitude in all areas of my life, especially when facing new challenges. Both opportunities have also allowed me to understand, and seek that balance between hard work, and efficiency, while also reaping the benefits of teamwork, and companionship with one’s coworkers. I sincerely believe that my skill set and background would be an asset to Ontario Power Generation, and my enthusiasm and teamwork would fit perfectly in the fabric of this company. Thank you for your time and consideration, and I look forward to meeting you and discussing how I can become a part of the OPG team.

Introduction Hacienda Luisita

Introduction Hacienda Luisita was once part of the holdings of Compania General de Tabacos de Filipinas, Sociedad Anonima, better known as Tabacalera, which was founded on November 26, 1881 by a Spaniard from Santander, Cantabria and Santiago de Cuba, Don Antonio Lopez y Lopez. He was the first Marques de Comillas and was famous for being an associate of the first Spanish Prime Minister with foreign blood, the Spanish-Filipino mestizo Don Marcelo Azcarraga y Palmero. His relative on his Spanish side, Ricardo Padilla, married Gloria Zobel y Montojo (younger half sister of Mercedes Zobel de Ayala de McMicking, largest Zobel owner in the Ayala group of companies) and was an aide-de-camp of Juan de Borbon, Count of Barcelona, father of the current King of Spain, His Majesty DonJuan Carlos de todos los Santos de Borbon y Borbon-Dos Sicilias. The estate was named after Antonio's wife, Luisa Bru y Lassus. Their son, Claudio Lopez, the second to hold the title , donated some of the profits to the Jesuits to create the Pontifical University of Comillas, a university outside Madrid. Lopez acquired the estate in 1882, a year before his death. Lopez was a financial genius who parlayed his work adventures in Cuba and Latin America into a steamship, companies and trading businesses. He was the most influential Spanish businessman of his generation and counted the Prime Minister and the King of Spain as his personal friends. Tabacalera was a private enterprise he founded with the sole intention of taking over the Philippine Tobacco Monopoly from the Spanish colonial government. This included the Hacienda Antonio (named after his eldest son), Hacienda San Fernando and Hacienda Isabel (named after his eldest daughter) in Cagayan and Isabela provinces where the legendary La Flor de Isabela cigar was cultivated. Tabacalera’s incorporators were the Sociedad General de Credito Inmobiliario Espanol, Banque de Paris which is now Paribas and Bank of the Netherlands which is now ABN-AMRO. The sugar and tobacco in the Philippines were the reason why the Lopez de Comillas family were able to donate such a huge pontifical university to the Jesuits on top of lavishing on their home, the Palacio de Sobrellano in Comillas and the Guell park (designed by Gaudi) in Barcelona. Don Alfonso Guell y Martos born in 1958, the fourth Marquis of Comillas, currently holds the title. He is also the Count of San Pedro de Ruisenada, the third to hold that title. Both are grandee status in Spain and as such can address the King as â€Å"mi primo† or â€Å"my cousin. Contrary to what was expected, Spanish-owned Hacienda Luisita did not languish when the Americans took full control of the Philippine government. In fact, Tabacalera as a whole experienced prosperous times because of the legendary sweet tooth of the Americans. With Cuban sugar not enough for their domestic market, the Americans tapped the Philippines for its sugarcane requirements. At one point during pre-war Manila times, Hacienda Luisita supplied almost 20% of all sugar in the United States. Luisita sugar became popular among Filipino (specifically Ilocano) expatriates in America just as much as Victorias sugar was popular among Manila’s elite circles back home. The Americans also brought the centrifugal-based machinery which doubled the production of the estate and therefore did not require the cane to be loaded by truck to Laguna to be squeezed in the haciendas there, including those of the Roxas y Zobel families. As this new technology swept in Luzon and the sugar mills consolidated, many wealthy families fell into foreclosure or combined their resources. Some of the brave few like Honorio Ventura (who paid for Diosdado Macapagal’s schooling), the De Leons, Urquicos, Lazatins and the Gonzalezes did just that— which is how PASUDECO came into being. Structurally, there was little change in the hacienda; Tabacalera y Compania positionedSpanish-Filipino and American-Filipino encargados and administradores to manage the vast estate. Like all haciendas and tabacaleras in the Philippines, the Hacienda Luisita continued to operate during the Japanese occupation. The Japanese were bent on ensuring that commodities such as sugar and rice be made available to the majority of the Filipinos, therefore avoiding any tempers of additional insurgencies and guerilla movements. The Spanish-Filipino administrators simply placed their subordinates, Japanese journeymen (who, like many impoverished Chinese immigrants from Fujian fled south to the Philippines for a better life) and Korean stevedores working as machinists in the centrifugal system, to the helm. This kept both the Japanese and the Spanish in good terms as both their interests were protected. As a matter of fact, even before World War II, the Tabacalera had in their payroll a good number of Japanese migrant workers doing odd jobs around Hacienda Luisita. (Before 1942, the Philippines was a first class colony in Asia while Hong Kong and Singapore were poor cities; Tokyo and Japan as a whole was relatively closed from the outside world then). When the Japanese Imperial Army marched into the country, these lowly migrant workers became valuable translators and managers. In conjunction with re-taking the Philippines from the Japanese, on January 25, 1945 General Douglas MacArthur moved his advanced headquarters forward to Hacienda Luisita. In the 1950s, the onset of the Hukbalahap rebellion led the Spanish owners of Tabacalera to sell Hacienda Luisita and the sugar mill Central Azucarera de Tarlac. Ramon Magsaysay, then president of the Philippines, blocked the sale of the plantation to the eager and wealthy Lopezes of Iloilo. During those times the brothers Fernando Lopez and Eugenio Lopez as well as their cousins were one of the wealthiest in all of the Visayas Islands, save for a few Chinese Filipino families in Cebu and Leyte, as well as the Familias Aliadas de Villegas, Teves, Lopez, y Rodriguez (a family with origins from Santander, Galicia, & Asturias; as well as China – Teves). Fearing the Lopezes might become too powerful after already owning Meralco, Negros Navigation, Manila Chronicle, ABS-CBN, various haciendas in Western Visayas and then the nearby PASUMIL consortium in del Carmen, Pampanga that they purchased from the Americans, the President offered the property to Jose Cojuangco, nicknamed â€Å"Pepe† through Magsaysay protege and Cojuangco's son-in-law, Benigno Aquino. Magsaysay also knew the Cojuangcos through his wife, Luz, of the prosperous Banzons, an old Chinese Filipino family. Unfortunately, President Ramon Magsaysay died in Mount Manunggal, Cebu in 1957. The sale was consummated in President Carlos P. Garcia’s term, a close ally of then Senator Ferdinand Marcos and five years from the day President Magsaysay offered the land. The Jose Cojuangcos were wealthy in land and bank holdings and in Philippine pesos. They were not wealthy in United States dollars which was closely regulated then by the Philippine Central Bank. In fact, Pepe and his wife Metring were not able to send Pepe’s younger brother Eduardo Sr. (Danding Cojuangco’s father) to the United States for treatment for the mere fact that they could not exchange their pesos to dollars. Eduardo Sr or Endeng Lalake later died of kidney failure. The Jose Cojuangcos acquired the property in 1958 through a loan from the Government Service Insurance System and a dollar loan from the Manufacturers Trust Company of New York, which was guaranteed by the Central Bank of the Philippines, with consent from Miguel Cuaderno, its governor. Pepe also reduced his stake in the Paniqui Sugar Mills, though he and his cousins still managed it on behalf of his aunt, Ysidra Cojuangco, the matriarch. Hacienda Luisita was the largest investment he ever made. With the ink barely dry, he appointed not his eldest son Pedro but his son-in-law Benigno Aquino Jr as administrator. Pepe and Ninoy introduced an almost social welfare state: free medicines and check up, scholarships to colleges, free education, free food and equitable shares to the harvest, free child care and nutrition, free burials, a village with housing earmarked for the farmers, even free gasoline to the tractoras. Like the Paniqui Sugar Mills, not a single workers’ strike was instigated during their administration. Pepe barely made any money from the Hacienda Luisita. Understanding that the value of the Luisita is in the farmers who till it, he chose to rehabilitate the Filipinos who before were almost slaves under the Tabacalera. He was able to sustain these losses due in part of his other more money making investments in the Bank of Commerce and First Manila Management which owned the Pantranco buses and the Mantrade group. As Ferdinand Marcos was elected for a second term in 1969, the reverse happened to Pepe. At Bank of Commerce, where he and his brother Juan â€Å"Itoy† Cojuangco and nephews Ramon Cojuangco(later of PLDT; son of relative Antonio Cojuangco Sr) and Danding Cojuangco (eldest son of deceased brother Eduardo Cojuangco Sr) each owned equitable stakes, the last three factions planned a coup d’ etat by toppling him from the presidency of the said bank. The three did not want Pedro (Pepe’s first born) to be bank president which was against the aging Pepe’s wishes. To avoid a scandal, Pepe Cojuangco sold his remaining shares in Bank of Commerce, almost equal to 28%, to his relatives. Thus Pepe lost his one of eventually three lifelines in nurturing the Hacienda Luisita. As the 1970s crept in and immediately after Benigno Aquino Jr imprisonment on false charges, Pepe’s business empire began to wane. He was unable to purchase new machines and new technology for the aging sugar mill that stands in the middle of the estate because of the government’s refusal to Pantranco’s appeals for higher charges as compared to its competitors who have since been permitted so. Business critics believed it was Marcos’s way of pressuring Pepe to influence his son-in-law from attacking him and his wife, First Lady Imelda Marcos(who recently built the Cultural Center of the Philippines and whom Ninoy labeled as the new Evita Peron). His close business associate in First Manila Management of the Pantranco / Nissan Philippines / Mantrade fame, Manuel Lopa, died in 1974. With his death, the FMMC-Mantrade companies lost their immunity from the Marcoses (Manuel was a close personal friend of Speaker Daniel Romualdez, Imelda's uncle). Ambassador Benjamin Romualdez, brother of Imelda, then coerced Pepe and his son-in-law, Ricardo â€Å"Baby† Lopa (Manuel’s son) into selling the collection of 38 companies under First Manila Management to him. Baby and his wife Teresita Cojuangco, together with Pepe and the rest of the Lopa heirs, had no choice but to sell. The second lifeline disappeared with this extortion. In 1976, First United Bank, the banking concern Pepe built on his own after his ouster from the family owned Bank of Commerce which he saved from bankruptcy decades ago, was sold for an amicable amount to his nephew, Danding Cojuangco, who was then close to President Marcos, with both mothers being Ilocanas notwithstanding. The poorest branch of the Cojuangcos, the Eduardo branch, has become the richest through the sheer genius of Danding. Though this third lifeline disappeared in good terms, the Jose Cojuangcos were left with nothing but a half-rehabilitated and barely earningwhite elephant of a hacienda. Practically all of his farm workers mourned his death. Many flooded his funeral Mass to see him off. Pepe Cojuangco died on August 21, 1976, five years from the day of the Plaza Miranda bombing. His wife, Demetria Sumulong-Cojuangco, died due to colon cancer (the same disease that killed daughter Cory Aquino). Both died disappointed and broken-hearted. Their children and grandchildren zealously took key positions in the holding company to save the hacienda from the creditors, all of whom wanted to slice Luisita away save for Chinabank of Binondo, who defied the anger of President and Mrs. Marcos by continuing to help them. Chinabank was partly owned by the Dee, Sycip and Lim families. With Ninoy and his wife Cory Aquino in exile in Boston, the remaining children took drastic steps in ensuring that the hacienda continued to exist and operate. To maximize the productivity of sugar and therefore profitability, a certain level of economy must be reached. Thus the Jose Cojuangcos tried their best to keep the Luisita in one piece. They refurbished and re-used old 1950s era farm machines and tools, doubled capacity production maintained low expenses. There were a lot of reasons why Luisita remained in Cojuangco hands. One, it helped that Danding Cojuangco was the de facto kingpin of Tarlac and his kind mother Josephine Murphy Cojuangco was still cordial to them. For Marcos to touch Hacienda Luisita he also would have to force Agrarian Reform into the Ysidra Cojuangco haciendas which were under the supervision of Danding Cojuangco. Thus, many haciendas around Luisita were hacked to smaller pieces such as those of the De Leons[disambiguation needed ],Escalers, Urquicos, Arrastrias, Quiasons and Gonzalezes[disambiguation needed ] but not those of the Cojuangco. Two, it helped that the price of sugar spiraled so high because of President Marcos and Roberto Benedicto manipulating the sugar prices primarily in Negros Occidental. Third, Ninoy Aquino was not in the Philippines lambasting President Marcos in the underground movements. For as long as the Marcoses heard less of Pepe’s son-in-law, the less government pressure there was on the Jose Cojuangcos. Most importantly, it helped that most of the farm workers who remember Pepe understood the frugality measures his children had to implement. On Pepe’s death anniversary and that of the bombing of Plaza Miranda, Ninoy Aquino was gunned down in broad daylight, August 21, 1983. Upon the installation of his wife, Cory, Pepe’s daughter, the property was folded into the Hacienda Luisita Incorporated established on August 23, 1988. [1] In compliance with the Comprehensive Agrarian Reform Program which at this time around did not exempt anyone whether or not they were close to President Marcos before, nearly 5,000 hectares of Hacienda Luisita were placed under astock distribution agreement between the landowners and farm workers. President Aquino wanted to make sure that all farmers’ rights are recognized. If the farmers agreed for a stock distribution agreement then the plantations would also remain intact. Many haciendas, including those assembled by Ysidra Cojuangco a century before, did not qualify or the farm hands there refused the offer. Thus, the majority of all Cojuangco lands disappeared while a Cojuangco was President of the Philippines. This caused a silent rift within the Cojuangco clan. All the lands where sugarcane and molasses were derived to feed the Paniqui Sugar Mills were hocked to appease the government program and those of the angry farm workers. Hacienda Luisita was saved by the perseverance of Cory’s siblings and the fact that most of the farm workers signed the agreement, counting that one day the life in Hacienda Luisita would be just as good as the time when Pepe and Ninoy used to managed it. However, development and new technology did not arrive in Cory Aquino’s term. She barred any relative from starting any new businesses. Furthermore, she forbade many among her siblings and cousins from retaking the family businesses lost in the 1970s unless it was sold back to them (as with the case between Romualdez selling back First Manila Management to the Lopa clan) or was awarded to them by the PCGG or Presidential Commission on Good Government. The old sugar mill in the middle of Luisita remained rickety and with holes in its roofs. After 1992, Cory Aquino stepped down from the Philippine presidency. That was also the time that elder brother Pedro â€Å"Pete† and sons Melecio â€Å"Mel† and Fernando â€Å"Nando† entered the hacienda hoping to make it profitable. Mindful of the farm workers, they instituted very slowly the fiscal reforms to achieve this goal. This partly explains why every year from 1988 until 2008 the Hacienda Luisita and itsCentral Azucarera de Tarlac posted hundreds of millions of losses. Only in 2009, buoyed by the huge demand for sugar and the unpredicted fluctuating prices of Brazilian sugar, did the family corporation post a profit. The various siblings stopped contributing money from their own non-hacienda corporations for the benefit of Pepe’s hacienda, which was a huge sigh of relief for them. On the other hand, the management style of the Pedro Cojuangcos lacked the charisma of the deceased Ninoy. His United States educated children, Mel and Nando, continue to strive to placate the needs of the farmers while balancing the budget. Sadly, when profit arrived so did the workers’ strikes. The unrest was blamed on the allies of current President Gloria Macapagal Arroyo who were shocked to see Cory Aquino joining anti-Arroyo rallies. Some blamed Danding Cojuangco since owning the hacienda would complement San Miguel and Ginebra’s ethyl, molasses and sugar needs. This was refuted by Danding himself and his cousins believe in him. In 2005, the Department of Agrarian Reform canceled the stock distribution agreement, citing that it had failed to improve the lives of more than 5 000 farmer beneficiaries. Hacienda Luisita Incorporated appealed this decision, but in May 2006, the Presidential Agrarian Reform Council rejected with finality the motion of Hacienda Luisita Incorporated to reconsider the revocation of the stock distribution agreement. However, the Supreme Court issued a temporary restraining order, stopping the Presidential Agrarian Reform Council from parceling out the land to the workers.

Microarray Student

In the cell cytoplasm, the ribosome reads the sequence of the Mrs. in groups of three bases to assemble the protein. D. What is the resulting primary sequence Of the protein you made in the interactive? Mennonite, Lucien, Aspartic Acid, Valise, Phenylalanine 2. Go to the â€Å"DNA Microfarad Virtual Lab† found at the university of Tutu's Learn-Genetics: Genetic Science Learning Center's website: http://learn. Genetics. Utah. Du/content/labs/microfarad/ 3. Click on the microfarad slide to begin. Then choose Chapter 2 â€Å"Measuring Gene Expression† and go on through Chapter 3 â€Å"The Experiment†.Complete the interactive a first time without writing anything down. 4. Answer the following questions as you work through the virtual DNA microfarad a second time. . What can scientists look for to see which genes are turned on in a particular cell? Provide an example. Scientists use DNA microfarad's that used techniques allowing the amount of Mrs. transcribed by each gene which allows scientists to determine which genes are expressed to a cell. To do so, they use PC to make copies of a gene and then DNA is placed on the microfarad.An example of this is when the DNA microfarad's can be used to detect single nucleotide polymorphisms (Snaps). B. Why are tissue samples from healthy and cancer cells taken from the same patient? Both samples are elected to be able to compare the cells, look at differences in gene expression in cells that have the exact genetic blue print. C. How is RNA separated from the rest of the tissue? In order to be separated, RNA samples are mixed with organic solvents, and then they run through a vortex and centrifuge. D. Describe the process used to isolate Mrs. from the other types of RNA. RNA is different from other types of RNA because it is the only type that has Poly-A tails, having several adenine amino acids at the end of the molecule. E. Explain how samples are marked. Cancerous cells are red and healthy cells are gre en. . Why is it necessary to make a CDMA copy? Why is Mrs. not used? RNA is not used because DNA is a more stable compared to RNA Making a CDMA copy is necessary to visualize the CDMA later on. This is possible because when you make the CDMA copy, you incorporate a fluorescent label in the molecule. G.Draw a diagram of the process of how labeled DNA copies are made. Label the important components of the process. Your diagram should be in color. Turned in. H. What does CDMA stand for and what was used as a template to create it? CDMA stands for complimentary DNA fragments. The reverse transcription of Mrs. allows for single stranded CDMA to be made. I. What happens once you apply the DNA from the two samples to the DNA microfarad? On the microfarad there are many identical copies of the same genes on certain spots, each spot represents a different gene. J. What does the red color indicate?The red color indicates genes that were induced by elevated CA. K. What does the green color ind icate? The green color indicates that the gene was strongly repressed by cancer cells. L. What does the yellow color indicate? The yellow color indicates that the gene was neither strongly expressed nor strongly repressed in cancer cells. . What conclusions can you make from microfarad data? Microfarad data is a collection Of DNA spots attached to a solid surface that's used to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. . Does every red spot mean that the genes in that spot are bad? Explain. Not every red spot is cancerous. Red spots on the merged image represent genes that have had their expression turned up or on in the cancerous tissues and are not expressed in normal cells, the they are not always cancerous. O. What are the limitations of DNA microfarad technology? The quality and amount of RNA remains a major challenge in the microfarad experiments. The amount of obtained tissue and the complexity of the t issue sample itself limit the quality and quantity of RNA that can be isolated.Therefore, clinical studies that are published using the microfarad approach are performed in settings where biological samples are abundant and easily obtainable. 5. Complete the Paper Microfarad analysis that comes with the kit. Part II: Smoking and Lung Cancer Grandpa Joe, Judy Smith's father, has been a smoker for the past thirty years. Last year, Grandpa Joe came down with a cold that turned into pneumonia. It took him more than a month to recover. The family is very concerned he is going to develop lung cancer.They heard about a study being conducted at the local hospital that is exploring lung-cancer associated genes in smokers and non-smokers. The family convinces Grandpa Joe to participate in the study in order to learn more about his risk for developing lung cancer. The study is investigating six genes thought to be involved with lung cancer using DNA microfarad technology. The researchers hope to compare gene expression of the six genes of interest between smokers and non-smokers in order to gain more knowledge of what causes a normal lung cell to become cancerous.You have been assigned to the study. Your first task is to learn more about the six genes of interest. Below are descriptions for the six genes of interest: Gene Name (and Symbol): Protein Function: Prediction: Gene l: Human carcinogenicity antigen (CANDACE) This gene codes for a protein that is located in the extracurricular matrix. This protein is involved with adhesion between cells and is thought to be a proto- nosecone and when over-expressed is an nosecone. Expressed in smoker. We predict that the CANDACE will progress to the form of a tumor or untie to harbor that potential.Gene 2: Surfactant protein B (SIFTS) This gene codes for an extracurricular protein. This protein enhances the rate of spreading and increases the stability of pulmonary' surfactant, a lipid-rich material that prevents lung collapse by lowering surface tension at the air- liquid interface in the alveoli of the lungs. Expressed in the Smoker. This gene can possibly progress to spread a higher production of cells or possibly a higher rate of cancer, maybe in the lungs. Gene 3: POP tumor suppressor (HTTP) This gene codes for a protein that is located in the mitochondria and in the nucleolus.This protein is involved with cell cycle checkpoints. This gene is a tumor suppressor gene and is thought to be the â€Å"Guardian of the Genome. † Suppressed in the smoker. I predict this gene is unlikely to get cancer as it monitors cell production, etc. To prevent tumors. Gene 4: CRY This gene codes for a protein that is located in the nucleus. The protein that this gene codes for is testis-determining factor (UDF) which initiates male sex determination. This protein has no function in lung cells. Not expressed in either. Non-smoker-?yellow.This gene probably determines whether or not you get cancer based on cell produc tion. Assume it contributes to getting cancer, unless in the lung cells, where there would be no increase or decrease in likeliness. Gene S: Stockroom IPPP (COPY AY) This gene codes for a protein that is located in the endoplasmic reticulum. The protein catalysts reactions involved in drug metabolism and synthesizes cholesterol, steroids, and other lipids. The expression of this protein is induced by some polycyclic aromatic hydrocarbons (PARS), some of which are found in cigarette smoke.Suppressed in smoker. This gene will contribute to causing cancer if deregulated because it controls cell signaling pathways. Gene 6: Clinical 3(SPEC) This gene codes for a protein that is located in the plasma membrane and extracurricular matrix. The gene controls cellular response to damage and may control cellular growth regulation and apotheosis. This gene is considered to be a tumor suppressor gene for lung cancer. Suppressed in smoker. This gene decreases your risk of cancer as it controls cel l self-destruction and responds to damage or advances in cell growth 6.Highlight or underline any interesting or important information about the function of each protein. 7. Predict how these genes will be expressed in a DNA microfarad of a smoker versus a non-smoker. Would you expect the genes to be induced in the smoker (more expressed), suppressed in the smoker (less expressed), not expressed in either the smoker or the non- smoker, or expressed the same in both the smoker and non-smoker? In a DNA microfarad, I would expect these DNA genes to have a bit of diversity. I think Gene 1 and 2 will be likely induced as they can contribute to overall cell production, 4 would be unaffected as Mr..Smith is testing for lung cancer, and 3, 5, and 6 to be suppressed as they work to control cell production and smoking destroys several of their functions. For example, Gene 6 works to control cell apotheosis– if smoking ruins the chances for a cell to destroy itself, production can get o ut of control. 8. Record your predictions in the Prediction column of the above table. Part Ill: Microfarad Wet Lab Now that you know more about the six genes of interest, your job is to perform a simulated DNA microfarad using tissue samples taken from Grandpa Joe and a non-smoker's tissue samples.

Legal Issue Essay Example | Topics and Well Written Essays - 750 words

Legal Issue - Essay Example In turn, a disability is (A) a physical or mental impairment that substantially limits one or more of the major life activities of such individual; or (B) a record of such impairment; or (C) being regarded as having such an impairment.[5] The EEOC defines "a mental impairment" as "any mental or psychological disorder, such as mental retardation, organic brain syndrome, Bi-Polar Disorder II, emotional or mental illness, and specific learning disabilities,"[6] for purposes of the ADA.[7] Thus, the question becomes whether this impairment substantially limits a major life activity.[8] Interacting with others is a major life activity under the ADA Interacting with others is indisputably an activity of central importance to daily life, and the EEOC has recognized it as a major life activity in its compliance manual.[9] Additionally, interacting with others is considered a major life activity under the Rehabilitation Act, and such recognition under the ADA would serve the legislative purpose of rectifying stereotypes about the mentally disabled.[10] Neither the Supreme Court nor the Second Circuit have addressed the issue of whether interacting with others is a major life activity.[11] The only circuit to have squarely addressed the issue has adopted it as a major life activity,[12] and no circuit has held that interacting with others is not a major life activity.[13] An Appellate Court has de novo review of a lower court's conclusion that the ability to interact with o thers is a major life activity.[14] In Toyota, the Supreme Court explained that "the term major life activity as used in the ADA, refers to those activities that are of central importance to daily life."[15] Because major life activity is not defined in the ADA, it should be construed according to its ordinary and natural meaning.[16] The plain meaning of the word "major" denotes an activity that is important and significant,[17] as well as basic. It is an "activity that the average person in the general population can perform with little or no difficulty."[18] Interact is defined by the dictionary to mean to "act upon one another."[19] Interacting with others is an important and significant function and "easily falls within the definition of 'major life activity.'"[20] - quoted properly referenced It is necessary for

Im One Archie Anderson Essay Example | Topics and Well Written Essays - 750 words

Im One Archie Anderson - Essay Example Environmental campaigns have also raised the need for alternative, renewable sources of energy within the society to conserve natural resources for posterity. The issue of environmental pollution caused by the burning of the fuels from this industry has led to an extensive criticism of the oil and gas industry in a harsh way. The I’m One Campaign sought to handle these crises by documenting all the benefits oil and gas to the American society. It reviews numerous aspects in which oil and gas are fundamental to the society to persuade them to support the industry. The advertisements states facts about the dependence of oil and the benefits it has to the American fraternity. Jobs, education and day to day activities within the American society get reviewed in the advertisement, and their relation to the existence of oil gets developed in the advertisement to enlighten the American consumers. (AmericanPetroleumInstitute) According to the campaign, 9.2 billion jobs in the US get maintained by the gas and oil industry. The capitalist nature of the US makes this statistic a welcome one as the citizens are in a constant mission to attain economic stability. The recession hit the country dearly leading to reduced job security, and thus any effort to improve the situation shall get immense support. This advertisement aims at this niche to enable the embracing of the oil and gas industry. The jobs that got covered in the campaign that get supported by the oil and gas industry were numerous. Among them were mechanics, power pricers, researchers, marketing coordinators, design engineers, field engineers, chemists, landmen and environmental, health and safety technicians. This long list in their advertisement brings out the quantity aspect of the benefits of the oil and gas industry. It goes to a personal level in naming the respective discipline apart from mentioning the staggering statistic of 9.2 billion

A Personal Analysis of Two images from UCL collections (Cultural and Essay

A Personal Analysis of Two images from UCL collections (Cultural and Historical Geography) - Essay Example This phenomenon is not a feature of photography, because a photo reflects exactly what a camera captures. For this reason photographs may even serve as evidence of certain historical events – they reflect the reality of the moment. On this matter James Curtis outlines that â€Å"Public and scholarly faith in the realism of the photographic image is grounded in a belief that a photograph is a mechanical reproduction of reality† (Curtis 2003, pp.1-2). Therefore, it is possible to assume that in most cases a photograph can give rather precise information about the people, places and events of a particular moment in time. For this reason it is interesting to investigate the social, cultural and historical context of photographs from different time periods to find out how these geographical aspects change through time, and how they are reflected by photography. Just like the ‘Group photograph of visit to Russia’, ‘Research into Heredity’ can be called a documentary photograph because it also tells a story of what was taking place around the moment of taking a picture. In particular, ‘Research into Heredity’ is a photo of a boating party. What is known is that the party took place sometime between 1880 and 1889, and the photograph of its participants was used by Sir Francis Galton as a part of his research into heredity and genetics. Most likely Sir Galton kept this photograph for scientific purposes. It is seen that some faces were cut out of the photo. So, it might be assumed that those cut out faces were then used by the photographer as an aid in his research of heredity. It can be seen that both, men and women, as well as some children, were present at the party. Most men are accompanied by a woman, so it is possible that men came to the party together with their families. Considering that many people in the photograph are relatives, the assumption